High performance liquid chromatography requires only a HPLC vial to be made into a solution, independent of the volatility of the HPLC vials, a wide range of mobile phases, and a wide variety of stationary phases, thus separating thermally unstable and non-volatile, dissociated and Non-dissociated and various molecular weight ranges of substances.
In combination with the HPLC vials pretreatment technology, the high resolution and high sensitivity achieved by HPLC make it possible to separate and simultaneously measure substances with similar properties, which can separate trace components in complex phases. With the development of the stationary phase, it is possible to complete the separation under conditions sufficient to maintain the activity of the biochemical.
HPLC has become the most promising method for solving biochemical analysis problems. Because HPLC has high resolution, high sensitivity, fast speed, reusable column, easy collection of effluent components, it is widely used in various fields such as biochemistry, food analysis, medical research, environmental analysis, inorganic analysis, etc. . The combination of high performance liquid chromatography and structural instruments is an important development direction.
Liquid chromatography-mass spectrometry has received widespread attention, such as the analysis of carbamate pesticides and polynuclear aromatic hydrocarbons; liquid chromatography-infrared spectroscopy has also developed rapidly, such as the determination of hydrocarbons in water by environmental pollution analysis, in seawater. The non-volatile hydrocarbons have led to new developments in environmental pollution analysis.