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A guard column, in a high performance liquid chromatograph(2ml Agilent wide opening vials), is installed between the injector and the analytical column, through the mobile phase and the sample solution.
Pre-capture can protect the column and extend the life of the analytical column by being firmly adsorbed by the analytical column and not being washed by the mobile phase.
The packing of the guard column is generally the same as the analytical column.
The length of the guard column is generally only 1-2 cm.
The guard column should first be removed from the analytical column during cleaning to prevent the eluent from entering the analytical column; then select the appropriate mobile phase for flushing.
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