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What are the characteristics of HPLC instruments?

Time:2018/08/10   Pageviews:0    Share:
   This is an article about Introduction of Autosampler vials. If you are interested, please contact us!

High pressure - pressures up to 150 to 300 kg/cm2. The HPLC column is depressurized by more than 75 kg/cm2 per meter.

High speed - flow rate is 0.1 to 10.0 mL/min.

Efficient – the number of plates can reach 5000/m. Separate up to 100 components simultaneously in a single HPLC column.

High sensitivity - UV detector sensitivity up to 0.01 ng. At the same time consume less sample.

HPLC has the following advantages over classical liquid chromatography:
Fast – usually one sample is analyzed for 15 to 30 minutes, and some samples can be completed in 5 minutes.
High resolution - the stationary phase and mobile phase can be chosen for optimum separation.
High sensitivity – up to 0.01 ng for UV detectors and 0.1 pg for fluorescent and electrochemical detectors.
Columns can be used repeatedly – using a single column to separate different compounds.


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