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The initial stage of liquid chromatography is to transport the mobile phase with a liquid level difference at room temperature and atmospheric pressure using a large diameter glass column, called classical liquid chromatography. This method has low column efficiency and long time (usually several hours). ). hplc vials High performance liquid Chromatography (HPLC) was developed on the basis of classical liquid chromatography and introduced gas chromatography theory in the late 1960s.
It differs from classical liquid chromatography in that the filler particles are small and uniform, and the small particles have high column efficiency, but cause high resistance. High-pressure liquid phase chromatography is required, so it is also called High Pressure Liquid Chromatography (HPLC). . Also known as high speed liquid chromatography (High Speed Li) due to the fast analysis speed. hplc vials
This is the end of the introduction of Liquid chromatography theory. I hope it can help you.