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HPLC is unique highly sophisticated chromatographic equipment used to separate compounds that are dissolved in a solution. This is the reason why it is called High performance LIQUID chromatography. Also it is termed as High PRESSURE liquid chromatography is due to its mobile phase. Its mobile phase moves due to gravity. Unlike other methods HPLC includes all three sections in chromatographic equipment which are separation, identification and quantification. HPLC is an instrument or a machine which has all these three sections itself. At present HPLC methods are used that are controlled by software.