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The main factors are the volume of the micro sample injection and the method of each sample injection.
The method of sample injection is very important. It is generally required to use the index finger to quickly enter the sample into the vaporization chamber. Usually the novice will be required to do this practice until the relative standard deviation of the five sample injection peaks of the novice is less than a certain value, such as 5 %.
The volume of each sample injection should be as uniform as possible. This is more stringent than the liquid phase requirement, because the liquid phase can be quantified by the loop, and the gas phase can only be injected through the syringe filters. This is especially true when the sample injection volume is small. .
In addition to the first time, there are also factors such as whether there are bubbles in the needle, whether the solvent is easily volatilized, and whether the sample is filtered.