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Cuvette

  • A cuvette (sample cell, absorption cell) is, in its basic level, fundamentally a test tube designed for use with optical analysis. Standard cuvettes are generally square or rectangular in cross section to avoid refraction artefacts. Depending on what part of the spectrum is under consideration, they may be made of quartz or optical glass although plastic cuvettes do exist for less demanding measurements.

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Products Features+

A cuvette (sample cell, absorption cell) is, in its basic level, fundamentally a test tube designed for use with optical analysis. Standard cuvettes are generally square or rectangular in cross section to avoid refraction artefacts. Depending on what part of the spectrum is under consideration, they may be made of quartz or optical glass although plastic cuvettes do exist for less demanding measurements.
The other types of cuvettes are more expensive than the plastic cuvette. It is disposable and will be eliminated once complete the spectrometric experiment to prevent risk from reusing cuvettes and damaging expensive quartz. Color and UV range can be analyzed by this type of cuvette. The smallest one are capable of holding 70µl, the medium size contains between 1.5ml and 3.0ml, and the biggest is for testing samples with 2.5ml or larger.[1]
Some cuvettes will be clear only on opposite sides, so that they pass a single beam of light through that pair of sides; often the unclear sides have ridges or are rough to allow easy handling. Cuvettes to be used in fluorescence spectroscopy[2] must be clear on all four sides because fluorescence is measured at a right-angle to the beam path to limit contributions from beam itself. Some cuvettes, known as tandem cuvettes, have a glass barrier that extends 2/3 up inside, so that measurements can be taken with two solutions separated, and again when they are mixed. Typically, cuvettes are 10 mm (0.39 in) across, to allow for easy calculations of coefficients of absorption. To measure the sample, the transparent side must be placed toward the light in spectrophotometer. For accurate measurement, these testing tubes should be cleaned and without any scratches.[3]
Cuvettes to be used in circular dichroism[4] experiments should never be mechanically stressed, as the stress will induce birefringence[5] in the quartz and affect the measurements made.



 
 

Technical Information+

Vial Finish Specifications

  • Andard Screw Thread Finish
  • "T" = Outer diameter of the thread
  • "E" = Inside diameter of the thread
  • "ID" = Inside diameter
  • "S" = Start of thread
  • "H" = Distance from top of finish to shoulder for closure clearance
  • Andard Screw Thread Finish
  • "T" = Outer diameter of the thread
  • "E" = Inside diameter of the thread
  • "ID" = Inside diameter
  • "S" = Start of thread
  • "H" = Distance from top of finish to shoulder for closure clearance

Screw Thread Finishes

  • GPI refers to the "Glass Packaging Institute"

  • The GPI is responsible for establishing and issuing standards for the types and finishes produced by American glass manufacturers.

  • GPI refers to the "Glass Packaging Institute"

  • The GPI is responsible for establishing and issuing standards for the types and finishes produced by American glass manufacturers.

    Typical GPI finishes found in the chromatography field are as follows:

  • GPI refers to the "Glass Packaging Institute"

  • The GPI is responsible for establishing and issuing standards for the types and finishes produced by American glass manufacturers.

  • GPI refers to the "Glass Packaging Institute"

  • The GPI is responsible for establishing and issuing standards for the types and finishes produced by American glass manufacturers.

Glass Technical Information

  • Borosilicate - A glass that is high in silicate and having at least 5% boron oxide.

  • Linear Coefficient of Expansion - Fractional change in length of glass per degree change in temperature.

  • Strain Point - Maximum temperature to which glass should be heated during use"

    Types of Glass:

  • USP Type - Pharmaceutical glass containers can be classified as USP Type I, II, III or NP.

  • Type I - Borosilicate glass represents the least reactive glass.

  • Type I glass has the least pH shift. (Lowest leaching characteristics) Coefficient of Expansion = 33 or 51 for Clear and 51 for Amber

  • Type II - is de-alkalized soda lime glass with higher levels of sodium hydroxide and calcium oxide.

  • Type III - soda lime glass - cannot be autoclaved.

  • Type NP - general purpose soda-lime glass used where chemical durability and heat shock are not factors.

  • Coefficient of Expansion = 92.

GLASS PROPERTIES

Color

Clear

Amber

Linear Coefficient of Expansion

33

51

Strain Point (Degrees Celsius)

515

535

USP Class Type

Type 1

Type 1

Light Protection

No

Yes

Plastic Properties

Type of Plastic Type of Plastic Type of Plastic Type of Plastic Type of Plastic Type of Plastic
Maximum use temperature, C/F 80°C/176°F 80°C/176°F 80°C/176°F 80°C/176°F 80°C/176°F
Maximum use temperature, C/F 80°C/176°F 80°C/176°F 80°C/176°F 80°C/176°F 80°C/176°F
Maximum use temperature, C/F 80°C/176°F 80°C/176°F 80°C/176°F 80°C/176°F 80°C/176°F
Maximum use temperature, C/F 80°C/176°F 80°C/176°F 80°C/176°F 80°C/176°F 80°C/176°F
Maximum use temperature, C/F 80°C/176°F 80°C/176°F 80°C/176°F 80°C/176°F 80°C/176°F
Note: Chart is general guideline only.
PP* = Some radiation resistant polypropylene resins available.
**Flexibility - Depends on thickness.

SEPTA SELECTION GUIDE

Materials Compatability Incompatability Resealability
Silicone Alcohols, acetone, ether, DMF,
DMSO
ACN, THF, chloroform, pyridine,
benezene, toluene, hexane, heptane
VERY GOOD
PTFE/Silicone
PTFE/Silicone/PTFE
PTFE resistance until punctured
then septa or liner will have
silicone compatability

 

VERY GOOD
VERY GOOD
Rubber
(Natural Butyl)

 

ACN, Acetone, DMF, alcohols
Diethylamine, DMSO, Phenol
Chlorinated solvents, aromatics,
hydrocarbons, carbon disulfide
EXCELLENT
Natural PTFE/
Natural Rubber
Butyl PTFE/Butyl
PTFE resistance until punctured
then septa or liner will have
rubber compatability

 

VERY GOOD

VERY GOOD
Viton*

 

Chlorinated solvents, benezene,
toluene, alcohols, hexane, heptane
DMF, DMSO, ACN, THF, pyridine
dioxane, methanol, acetone
VERY GOOD 

 

Process Flow Diagram+

  • STEP 01

    The workers clean the tubing with cloth

  • STEP 02

    The workers plug the tubing into machine for making vials

  • STEP 03

    The vials are transferred to QC for Physical Test

  • STEP 04

    The workers put the tested vials into one big package (500-800pcs/pack)

  • STEP 05

    The workers get the vials from big package and put the vials into one special tray.

  • STEP 06

    Put the tray with vials into the Water injection machine

  • STEP 07

    The vials in tray will be transferred to next step for Ultrasonic oscillations.

  • STEP 08

    The vials in tray will be transferred to Jilt water machine.

  • STEP 09

    The vials in tray will be transferred to Infrared drying case.

  • STEP 10

    The workers will collect the vials after vials are dry.

  • STEP 07

    The vials in tray will be transferred to next step for Ultrasonic oscillations.

  • STEP 11

    The workers will check all the vials inclouding the bottom neck ,bottom ,inerts.

  • STEP 12

    The workers will pack 100pieces vials into one package.

  • STEP 13

    The workers will send the package to sealing machine for packing.

FAQ+

WHAT TO CONSIDER WHEN SELECTING AN AUTOSAMPLER VIAL

Autosampler Compatibility

Not all autosamplers are alike. Some utilize robotic arms to pick up a sample vial; some use tray rotation while others move the sampling needle to the respective vial coordinates. The dimensions of autosampler vials vary. Most autosamplers are equipped with trays that use 12x32mm vial ...Not all autosamplers are alike. Some utilize robotic arms to pick up a sample vial; some use tray rotation while others move the sampling needle to the respective vial coordinates. The dimensions of autosampler vials vary. Most autosamplers are equipped with trays that use 12x32mm vial ...

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WHAT TO CONSIDER WHEN SELECTING AN AUTOSAMPLER VIAL

Autosampler Compatibility

Not all autosamplers are alike. Some utilize robotic arms to pick up a sample vial; some use tray rotation while others move the sampling needle to the respective vial coordinates. The dimensions of autosampler vials vary. Most autosamplers are equipped with trays that use 12x32mm vial ...Not all autosamplers are alike. Some utilize robotic arms to pick up a sample vial; some use tray rotation while others move the sampling needle to the respective vial coordinates. The dimensions of autosampler vials vary. Most autosamplers are equipped with trays that use 12x32mm vial ...

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