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The amount of sample in the HPLC vials depends on what you do. If it is a headspace analysis, the upper limit of the sample volume is 80% of the volume of the vial so that there is enough headspace for sampling. Often the sample volume is 50% of the sample volume; if the liquid injection is not too strict, as with the LZ sample, a vial with an inner cannula can be used. The syringe insert depth of the autosampler is adjustable. Normally it defaults to a value. If you lower the value, the syringe will go down.
Or take ACE ultrasonic cleaning first, and then dry it in a potassium permanganate wash solution. However, the lotion is difficult to get in and it is not safe. In the EPA analysis, there is also ultrasound, dried and then taken to the muffle furnace for 450 degrees for three or four hours, but the bottle is easier to break.
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