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A chromatography hplc vials workstation is a system that extends a general-purpose computer from both hardware and software to handle the chromatographic signal data. The chromatography workstation consists of hardware and software.
1. Hardware: refers to the signal collector, also known as the analog/digital (A/D) converter, hplc vials which converts the analog signal output from the chromatograph detector (ie, the level signal) into a digital signal that the computer can receive. It is the sampling data), which acts as a bridge between the computer and the chromatograph. If the chromatograph itself is capable of outputting a digital signal (that is, the analog/digital converter is built inside the instrument), then such hardware is not needed. However, instruments that can directly output digital signals also retain the analog output signal of the Analog port to support the workstation software with its own analog/digital converter.
As we all know, chromatography is a very sophisticated analytical instrument, ranging from constant to trace, even to trace amounts, spanning 6-7 orders of magnitude, and the workstations that are compatible with it should reach a level higher than the instrument, in order to be objective and realistic. Reflect the results of the analysis. The signal collector has the same reproducibility and linearity as the chromatograph itself. In general, these indicators are only better than the indicators of the instrument itself, so as not to have a negative impact on the performance of the entire system. If the performance of the workstation's matching collector is lower than the performance of the instrument itself, the instrument with high sensitivity and good precision will experience a decline in overall performance. As described in the book "Gas Chromatography Instrument System" (Chemical Industry Press, January 2007, 1st edition), "In the processing of chromatographic data,hplc vials it was found that due to poor data processing performance, the analysis of hard matching would be made. The system and the best way to optimize the chromatographic separation conditions to obtain trace component peak distortion. In order to achieve the purpose of analysis and obtain accurate quantitative analysis results, the data processing device should be selected as the top product. Practice proves: a poor quality data processing device When doing data processing, it is a help because the performance of the instrument does not match the analysis requirements of the instrument."
At present, most A/D chips used in signal collectors are based on delta-sigma conversion, but some are based on inexpensive V/F conversion. The former can maintain high resolution, good reproducibility and linearity over the entire range of chromatographic signals, while the latter often compensates for its performance by means of range switching, software interpolation or software removal of noise. The resolution, reproducibility, and linearity are not consistent throughout the chromatographic signal range, and it is easy to leak peaks during capillary analysis or microanalysis, even when performing constant analysis. The primary factor affecting the measurement accuracy of the collector is noise. There are usually several ways to reduce noise, such as selecting high-precision devices, rationally designing to avoid interference, and removing noise by software. But the software method will affect the authenticity of the measured data. Just as a photo of 2 million photos cannot be processed into 8 million-phase photos, high-precision measurement can only rely on good hardware. Assuming that the collector actually achieves 21-bit accuracy, such a collector can accurately detect a change of 2,500,000 μV / 221 or 1 μV in the range of -1.25V to 1.25V (currently only a few manufacturers in China can really do this) a little). The easiest way to test the minimum resolution of a collector is to short its signal lines positively and negatively, and to set the vertical scale scale on the workstation screen to a minimum. The observed amplitude of the baseline fluctuation is collected for this purpose. The minimum resolution of the device (as shown in the figure below), because the limit state of the components and circuits has been reached, the baseline cannot be jumped up and down at the minimum resolution. If there is no fluctuation at the baseline, the workstation is in software. Cover up its real resolution.
On the other hand, because the workstation is the place that needs the most contact in the whole set of chromatography system, it will have a direct impact on the daily work efficiency, so the software performance of the workstation is also very important. The software interface is not as complex as possible. Conversely, the simpler and easier-to-use software requires more time and effort on the developer's mind, saving the user time.
"Gas Chromatography Instrument System" pointed out that "due to the characteristics of the chromatographic method itself, the complexity of the post-processing process of its analytical data is determined, so the availability of the analytical results is largely related to the reliability and data processing of the data processing device. It's about performance. It used to be said that the design of the chromatograph is the heart of the detector. The key to the chromatographer's use of the instrument is to choose a good column. Now it is important to emphasize accurate and reliable quantitative results. The heart part is the function of the data processing device. Even the best chromatograph and analysis method, if you do not choose a chromatographic data processing device, will not get accurate and reliable results." Occasionally, users buy high-end instruments at any cost, but they think they are the same when they are supporting workstations. It is no wonder that each manufacturer’s own indicators are internationally advanced, even in already cheap domestic products. According to word of mouth, the price is selected (in fact, the price of even the best products in domestic workstations is generally only a fraction of that of similar foreign products), and it is found in practical use that such as reproducibility declines, no small peaks, Low-level problems such as increased noise and even software crashes have caused continuous troubles for weekday analysis. Therefore, before purchasing a chromatography workstation, you should spend some time to understand the true performance of the product.
