How to avoid debris and vacuum problems caused by the PTFE septum?

Time:2018/11/09 Pageviews:4 Share:

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Debris

Debris is generated when the diameter of the needle is too large relative to the septum or the septum material cannot withstand multiple injections. At this point, the septum material will fall into the vial and contaminate the sample. Here's how to prevent debris:

1 Select a PTFE-lined septum to prevent the septum material from entering the sample

2 Make sure the needle is not damaged. Consider using a side hole needle instead of a tapered needle.

3 Using pre-punched septa almost completely eliminates debris

produce

Sometimes aspirating a sample from a sealed vial can result in a vacuum. Some simple steps can prevent this from happening:

1 Use a pre-punched septum to help balance the pressure on both sides of the cap

2 Do not fill too full

3 Do not exceed 50 μL in one injection (from a 2ml HPLC vials)

2ml snap HPLC vials

This is the end of the introduction of How to avoid debris and vacuum problems caused by the PTFE septum? I hope it can help you.