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About the regeneration of the HPLC column

Time:2018/11/26   Pageviews:6    Share:
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In chromatographic experiments, the column plays a considerable role 9mm  HPLC vials manufacturer. The column consists of a column tube and a stationary phase. It is divided into a packed column and a capillary column according to the thickness of the manifold and the filling of the stationary phase. The important indicators for testing the column are generally the degree of separation, the number of theoretical plates, the peak width, the peak shape, etc., and the degree of separation and the number of theoretical plates should be more important. Because the column is a consumable, as the time of use or the number of injections increases, a curve appearing on the recorder is called a chromatographic peak. >The peak height of the chromatogram is lowered, the peak width is increased, or the shoulder peak appears. Generally, the efficiency may be decreased 9mm  HPLC vials manufacturer.

(1) Regeneration of the reversed-phase column: sequentially using 20-30 times the column volume of methanol: water = 10:90 (V/V), acetonitrile, isopropanol as the mobile phase to wash the column, and then reverse Rinse the column sequentially.

(2) Regeneration of the normal phase column: The column is washed with n-hexane, isopropanol, dichloromethane, and methanol as the mobile phase in the order of 20-30 times the column, and then the column is washed in the reverse order 9mm  HPLC vials manufacturer. It is important to note that the above solvents must be dehydrated strictly.

This is the end of the introduction of About the regeneration of the HPLC column. I hope it can help you.


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