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a. Unpack the column and confirm the column type, size, date of manufacture, and solvent stored in the column.
b. Unscrew the sealing plugs at both ends of the column and put them back in the box for later use.
c. According to the flow direction indicated on the column tube, connect the inlet end of the column to the inlet of the injection valve through the connecting pipe (if the conditions permit, it is recommended to use the guard column in front of the column); the outlet of the column is connected to the detector. The connecting pipe is a stainless steel pipe having an outer diameter of .57 mm and an inner diameter of 0.1 mm to 0.3 mm. Both ends of the connecting tube have a hollow screw and a sealing ring for sealing. When taking over, be sure to try to reduce the dead volume outside the column. After the connecting tube passes through the hollow screw and the pressure ring, insert it as far as possible into the end, then tighten the hollow screw clockwise until it stops, then use the wrench to continue to screw 1/4-1/2 turn clockwise. Remember not to use too much force. If the column is leaky after being pressurized by the mobile phase, use a wrench to turn it 1/4 turn clockwise until it is not leaking.
This is the end of the introduction of Column installation. I hope it can help you.