HPLC Systems Focus on New Directions

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High performance liquid chromatography (HPLC) was first described in a landmark paper by Csaba Horvath and Seymour Lipsky in 1966, followed by the 1967 debut of the Waters ALC-100. Over the next thirty-plus years HPLC evolved significantly but incrementally to meet the analytical needs of a relatively new science—therapeutic biotechnology.
The next big breakthrough came, also from Waters, with the 2004 introduction of 2004 UltraPerformance LC (UPLC), which was based upon sub 2-micron porous particles and three to four times the pressure of standard LC, and much smaller flow rates.

Detection modes have also helped to shape HPLC’s evolution. Mass spectrometry has had the largest impact since it assumes a good deal of the separation and quantitation requirements. “Methods take less time because of this,” McGinley tells GEN. “One no longer needs to get baseline resolution of two closely eluting compounds as the MS can be used to get good gaussian peaks, even for compounds eluting at the same time. Also, column dimensions have continued to drift towards 2.1mm ID versus 4.6mm ID to exploit the LC/MS compatibility that 2.1mm ID provides.”

Improvements in HPLC are sure to continue, but perhaps not from the direction one would assume. “New HPLC systems seem to be more focused on regulatory compliance and fitting into a more automated laboratory environment, and less on specific performance improvements. Inert, metal-free systems seem to be a common feature in new LC systems, mostly due to the desire for better recovery and performance for ‘sticky’ biomolecules.”

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This is an article about HPLC Systems Focus on New Directions. If you are interested, please contact us.